My Profile

Dr. Siya Ram
Faculty Associate, School of Biotechnology Gautam Buddha University, Gr. Noida, UP.

Research Areas

Molecular Microbiology

Contact Information

School of Biotechnology,
Gautam Buddha University,
Greater Noida,
Gautam Budh Nagar-201306 (U.P)
India.
Phone: 09310732403/09458654007
Email: siyaram1@rediffmail.com

Academic Education

PhD in Biochemistry from Dr. RML Avadh University, Faizabad, . Dissertation title “STUDIES ON MOLECULAR DETECTION OF WATERBORNE PATHOGENIC MICROORGANISM”

Master’s, in Biotechnology from Banaras Hindu University, Varanasi. Dissertation title “BACTERIAL ENDOPHYTES IN AGRICULTURAL CROPS SCREENING IN RICE”

Bachelor’s in Industrial Microbiology from Dr. RML Avadh University, Faizabad. (BIOSCIENCES, SPECIALIZATION WITH INDUSTRIAL MICROBIOLOGY)

Research Interests

1. The biology of antimicrobial drug tolerance and drug discovery including biofilm and persister cell.
2. Development of nucleic acid based techniques (Real-time and LATE PCR probe array, DNA biosensor/biochip, and Nano probe).
3. State-of-the-art techniques to understand the ecology of pathogens using bioinformatics and metagenomic approaches.
4. Diagnosis, gene-expression array and mutation /SNP array of cancerous cells.

Teaching Activities

Genetic Engineering, General Microbiology and Nanobiotechnology

Appointments

2009 Faculty Associate: School og Biotechnology Gautam Buddha University, Gr. Noida, Gautam Budh Nagar, UP.
2008 Senior Research Scientist: ARA HealthCare Pvt. Ltd. Plot No. 10, Electronic city, Sector 18, Gurgaon-122015, HARYANA, (Feb, 4).
2004 Senior Research Fellow: Industrial Toxicology Research Center, Lucknow, (Dec, 15).
2002 Junior Research Fellow: Industrial Toxicology Research Center, Lucknow, (Dec, 16).

Awards and Honors

2005 Best Oral Presentation Award (ICTEOH - Nov, 14) held at Industrial Toxicology Research Center, Lucknow, India.
2005 Best Poster Presentation Award (ICTEOH - Nov, 14) held at Industrial Toxicology Research Center, Lucknow, India.
2004 Senior Research Fellowship (CSIR, India, NET- SRF, Dec, 15).
2001 Junior Research Fellowship (CSIR, India, NET- JRF, Dec, 16).
2000 National Eligibility Test (CSIR, India, NET-LS, Dec, 23).

Professional Memberships/ Associations

2007 Life Member - Biotech Research Society of India (BRSI).
2006 Life Member - Society of Toxicology India (STOX).
2005 Student membership - Indian Society for Cell Biology (ISCB).

Training/ Workshop Attended

2007 National Workshop on Current Trends in Computing and Bioinformatics, Feb, 2), held at University Institute of Engineering and Technology, C.S.J.M. University Kanpur, India.
2005 Bioinformatics Analysis using Accelrys GCG and SeqLab Workshop, Dec, 15, held at Institute of Microbial Technology, Chandigarh, India.
2004 Training Workshop on Research Methodology and Statistical Method in Biomedical Research, July, 26, held at Industrial Toxicology research center, Lucknow, India.
2004 Training Workshop on Scientific Communication, July, 24, held at Industrial Toxicology research center, Lucknow, India.
2005 Attended continuing education courses, ICTEOH, Nov, 14, held at Industrial Toxicology research center, Lucknow, India.
1994 Summer training at Parag Dairy Industry from Lucknow (Microbiological quality control and effluent treatment plant sections).
1996 Summer training at Distillery Industry from Faizabad, (Microbiological quality control and effluent treatment plant sections).
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Publications 14

Conference proceedings/Papers presented

(A) International

1. Shanker, R.; Ram, S.; Lata, P.; Vajpayee, P.; Jyoti, A.; Patel, C. B.; Dwivedi, P. D. (2: 1 S - 42) Pathogen Detection: PCR Probes to Nano-Probes! (2008). International Conference Nanotoxicology (Feb., 5 - 7th, 2008), IITR, Lucknow, India.

2. Shanker, R.; Vajpayee, P.; Ram, S.; Lata, P.; Jyoti, A.; Patel, C. B.; Dwivedi, P. D. (SII pp12 1 - 3), PCR Probes to nano-probes: Towards making pathogen detection fast and simple!, International Conference on EMSI (Jan, 1 - 3rd, 2008 ), Aligarh Muslim University, Aligarh, India. Aligarh.

3. Shanker, R.; Ram, S. (30, A1-OP-06), Detection of diarrheagenic Escherichia coli serotypes in water by TaqMan probes, International Conference on Toxicology, Environmental and Occupational Health (Nov., 14 - 17th, 2005), Lucknow, India.

4. Lata, P.; Ram, S.; Vajpayee, P.; Shanker, R. (46, S8-PP-07), Probing enterococci in surface waters: Plate to PCR, International Conference on Toxicology, Environmental and Occupational Health (Nov., 14 - 17th, 2005), Lucknow, India.

5. Ram, S.; Tripathi, U.; Seth, P. K., Shanker, R. (121, pp.115), Phenotypic and genotypic characterization of E. coli strains in water resources, International Symposium on Molecular Toxicology and Environmental Health (Nov., 4 - 8th, 2003), Lucknow, India.

(B) National

1. Ram, S.; Vajpayee, P.; Shanker, R. (P-62), Rapid culture independent quantitative detection of enterotoxigenic Escherichia coli in surface waters by real-time PCR with Molecular beacon, 5th Annual Conference of Biotechnology Society of India (Nov., 17 - 19th, 2007), ITRC, India.

2. Ram, S.; Jyoti, A.; Lata, P.; Vajpayee, P.; Shanker, R. (92, O/IV/1), Probing pathogens in waste waters: real-time PCR probes, National Conference on Scope and Applications of Microbes in Agriculture and Environment (Feb., 19 - 21st, 2007), Kanpur, India.

3. Ram, S.; Singh, R. L.; Shanker, R. (120, PII-58), Detection of enterohemorrhagic E. coli in water: In -silico probing for ‘real’ real-time PCR probes, 75th Annual Meeting of the Society of Biological Chemist India (Dec., 8 -11th, 2006), Delhi India.

4. Ram, S.; Lata, P.; Vajpayee, P.; Shanker, R. (140, PP-85), Diversity of ‘indicator’ bacteria in river Gomti water: drug resistance and virulence genes, XXIX All India Cell Biology Conference and Symposium on Gene to Genome: Environmental and Chemical Interaction (Jan., 17 - 20th, 2006), Lucknow, India.

Research Work

My research work mainly focused on the development of rapid, reliable and highly sensitive culture independent real-time PCR technique for quantitative detection of water and food-borne pathogens in real-life situations. During last five and half year research period, I have acquired working experience in generating plasmid, antimicrobial drug resistance and virulence gene profiles of entero/shiga toxin producing E. coli (ETEC/STEC) isolates from surface and potable water resources. I have also explored the area of pathogen detection by designing and evaluation of molecular probe chemistries (Molecular beacon, TaqMan, FRET and Scorpion probe) using real-time PCR, LATE PCR and Nano probe approaches.

My doctoral research focuses on the several parameters including microbial water quality analysis viz., quantitative enumeration of total coliforms, faecal coliforms and faecal streptococci from surface and potable water resources using MPN/MFT technique, isolation and biochemical characterization of pathogenic bacteria (E. coli, Enterococcus and Salmonella sp.). The core component of our research work includes determination of antimicrobial drug resistance and virulent gene signatures of STEC/ETEC isolates from surface and potable water resources. I am also conversant with retrieval of housekeeping/virulence gene sequences from GenBank database, sequence analysis (clustalW), designing and In-Silico validation of real-time probe chemistries (Molecular beacon, TaqMan, FRET and Scorpion probe). Additionally, I have compared four real-time probe chemistries on the basis of Tm and free energy ΔG values of cross dimer, hairpin and self-dimer in term of reproducibility, specificity and sensitivity. In order to facilitate this approach, I have established a rapid culture-independent real-time PCR assays for the detection of STEC/ETEC in different water resources. Furthermore, we have also demonstrated the interaction of ETEC with root mats of E. crassipes from non-point sources of surface water by using molecular beacon approach.

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