Bamboo and Rattan/Bamboo/Course-1 Unit-6
6.1: Conventional methods(offsets, rooting cuttings, layering, proliferation etc.)
6.2: Micro-propagation
(Taken from: Training Manual : "The INBAR-UPLB-ERDB International Training on Bamboo Propagation: Techniques, Utilization and Program Development"
Micro-Propagation of Bamboo, Produced by Alfinetta B. Zamora )
SECTION 1. MICROPROPAGATION PROTOCOLS
What is micropropagation?
High tech stuff?
Can we micropropagate bamboo?
What can you use for establishing cultures?
How can you approach micropropagation?
SECTION 2. "WHAT DO I NEED TO DO MICROPROPAGATION?"
Activities and suggested equipment v'''is a vis 'space
Other considerations
Chemicals and other supplies
SECTION 3. MAKING THE MOST OF SEEDS AND SEEDLINGS
A. Collecting seeds and seedlings
Seeds
Seedlings
B. Processing of the inflorescences for seeds
C. Storage of seeds
Testing for Seed Viability
D. Aseptic germination
1. Disinfection
2. Excision and inoculation of embryos
E. Multiple shoot formation from nodes of aseptic seedlings
1. Inoculation
2. Propagation of Calli/Somatic Embryoids
3. Plant Regeneration
H. Wildings or Nursery-Grown Seedlings
I. '''Invitro '''Rooting
SECTION 4. I WANNA BE JUST LIKE YOU!
"When I grow up, I will be just like you".
I. GROUND CORMS
A. Collecting ground corms
B. Handling of corms for culture
C. Storage of corms
D. Disinfection of corms
E. Preparation and inoculation of explants
F. Subculture and selection of calli
G. Propagation of compact-type calli
H. Shoot and shoot-like structures and plant regeneration
II. UNEXTENDED AND EXTENDED BRANCHES
A. Collecting branches
B1. Preparation of nodes and disinfection
B2. Preparation of unextended branches and disinfection (Fig. 4.9)
C1. Callus cultures
C2. Multiple shoot formation from nodes (Fig. 4.10)
D. '''In vitro 'rooting
Section 5. Getting Ready for the Real World
A. Acclimatization of plantlets for potting out
If humidity is a problem:
If you have a growth chamber:
B. The nursery
Potting out and nursery care
Procedures
SECTION 6. TRY MINI-CLUMP DIVISION
Advantages of tissue cultured plants and mini-clumps
Limitations of mini-clumps
How is mini-clump division done?
Micro-enterprises using mini-clump division
Thought For the Section:
Section 7. Making up for lost forests
A. Preparation of plants, transport and field planting
B. Degraded soils along the slopes of a mountain
C. Planting in Lahar
SECTION 8. HELP ME OUT!
A. SECURING THE INITIAL PLANTING MATERIAL
B. LABORATORY-BASED ACTIVITIES
C. NURSERY-BASED ACTIVITIES
D. FIELD ACTIVITIES
