Acharya Narendra Dev College/Biology Protocols

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Work in progress, expect frequent changes. Help and feedback is welcome. See discussion page.

The University of Delhi has recently revised its undergraduate syllabus - introducing some exciting experiments in the practicals of the Biology Course of the B.Sc. Programme. We, at Acharya Narendra Dev College believe in free access to education. That's why we have made protocols of various experiments available to all

Please do contact Principal if you have any comments or suggestions. We are open to improvement!!

Over time, on this page, we intend putting up protocols for all the Biology experiments.

Contents

Protocols for Practicals of B.Sc. Programme

B.Sc. Programme I yr - Foundation Course

  1. Isolation of Genomic DNA from E.coli.
  2. Plasmid DNA Isolation from E.coli.
  3. Quantification of DNA by spectrophotometer and estimation of absorption maxima.
  4. Isolation of genomic DNA from plant tissue by spooling method
  5. To separate DNA by Electrophoresis
  6. To Quantify DNA using Agarose gel electrophoresis
  7. To study mitochondria in striated muscle cells using vital stain Janus Green B.
  8. To study animal cells by making temporary mounts.

B.Sc. Programme II Yr

  1. Separation of single and double strand DNA
  2. Isolation of mononuclear cells by Ficoll-Paque PLUS
  3. To separate serum from blood
  4. To conduct Ames test for screening substances for mutagenicity

B.Sc. Programme III Yr

  1. To perform Western blotting
  2. To perform Southern blotting
  3. To perform Southern blotting
  4. Useful Bioinformatic sites on Internet for gene and protein databases,genome and organism specific databases.
  5. Homology search using 16srRNA sequence and performing multiple sequence alignment.
  6. Construction of phylogenetic trees.


Work in progress, expect frequent changes. Help and feedback is welcome. See discussion page.
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