Bamboo and Rattan/Bamboo/Course-1 Unit-6

6.1: Conventional methods(offsets, rooting cuttings, layering, proliferation etc.)

6.2: Micro-propagation

(Taken from: Training Manual : "The INBAR-UPLB-ERDB International Training on Bamboo Propagation: Techniques, Utilization and Program Development"

Micro-Propagation of Bamboo,  Produced by Alfinetta B. Zamora )

Micropropagation

'''SECTION 1. MICROPROPAGATION PROTOCOLS'''

What is micropropagation?

High tech stuff?

Can we micropropagate bamboo?

What can you use for establishing cultures?

How can you approach micropropagation?

'''SECTION 2. "WHAT DO I NEED TO DO MICROPROPAGATION?"'''

Activities and suggested equipment vis a vis 'space'

Other considerations

Chemicals and other supplies

'''SECTION 3. MAKING THE MOST OF SEEDS AND SEEDLINGS'''

A. Collecting seeds and seedlings

Seeds

Seedlings

B. Processing of the inflorescences for seeds

C. Storage of seeds

Testing for Seed Viability

D. Aseptic germination

1. Disinfection

2. Excision and inoculation of embryos

E. Multiple shoot formation from nodes of aseptic seedlings

1. Inoculation

2. Propagation of Calli/Somatic Embryoids

3. Plant Regeneration

H. Wildings or Nursery-Grown Seedlings

I. Invitro Rooting

'''SECTION 4. I WANNA BE JUST LIKE YOU!'''

"When I grow up, I will be just like you".

I. GROUND CORMS

A. Collecting ground corms

B. Handling of corms for culture

C. Storage of corms

D. Disinfection of corms

E. Preparation and inoculation of explants

F. Subculture and selection of calli

G. Propagation of compact-type calli

H. Shoot and shoot-like structures and plant regeneration

'''II. UNEXTENDED AND EXTENDED BRANCHES'''

A. Collecting branches

'''B1. Preparation of nodes and disinfection'''

'''B2. Preparation of unextended branches and disinfection''' (Fig. 4.9)

'''C1. Callus cultures'''

'''C2. Multiple shoot formation from nodes''' (Fig. 4.10)

D. In vitro 'rooting'

'''Section 5. Getting Ready for the Real World'''

A. Acclimatization of plantlets for potting out

If humidity is a problem:

If you have a growth chamber:

B. The nursery

Potting out and nursery care

Procedures

'''SECTION 6. TRY MINI-CLUMP DIVISION'''

Advantages of tissue cultured plants and mini-clumps

Limitations of mini-clumps

How is mini-clump division done?

Micro-enterprises using mini-clump division

Thought For the Section:

'''Section 7. Making up for lost forests'''

A. Preparation of plants, transport and field planting

B. Degraded soils along the slopes of a mountain

C. Planting in Lahar

'''SECTION 8. HELP ME OUT!'''

A. SECURING THE INITIAL PLANTING MATERIAL

B. LABORATORY-BASED ACTIVITIES

C. NURSERY-BASED ACTIVITIES

D. FIELD ACTIVITIES