Histology Methods

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Introduction

Histology is usually studied under the microscope. This means that one can work with tissue pieces small enough that permit the light to pass through them. Tissues are generally transparent, and thus the only way to see them under the microscope is either by taking advantage of some of light's physical properties (such as diffraction) or by staining the tissue with specific dyes.

Microscopy

Light

Epifluorescence and Confocal Microscopy

Electron Microscopy

Special Methods

Sample Preparation

Fixation

Embedding

Cutting

Staining

Fixation Methods

Embedding methods

Cutting Methods

Staining Methods

Classical Staining Methods

Hematoxylin and Eosin staining

Immunocytochemistry